Channel Based SPR (Biacore™)
Microfluidic channel based surface plasmon resonance (SPR) is one of a number of critical biochemical and biophysical technologies used by NovAliX to validate and to characterize screening hits. The highly sensitive SPR systems Biacore™ 3000 and T200 provide useful information concerning the binding of small molecules to target proteins immobilized on the SPR surface.
The NovAliX chemical microarray SPR imaging platform is a primary screening technique, generating hits that are subsequently verified as useful, specific binders to the target. Channel based SPR has a lower sample throughput than the microarray SPR but delivers detailed information including selectivity, affinity and kinetics of compound/target interaction (e.g. on-off rates). The specificity of any binding may be confirmed by competition assays with confirmed active site binders. SPR provides a very useful way of ranking of hits from a primary screen, greatly facilitating the hit to lead process.
Characterization by channel SPR is provided by NovAliX as a stand-alone service for the characterization of interactions with numerous applications:
- Antibody/antigen interactions
- Antibody ranking – scouting analysis
- Confirmation of direct and specific interactions
- Concentration analysis
- Epitope mapping
- Enzyme/substrate interactions
- Protein/DNA interaction
- Protein/protein interaction
- Receptor/ligand interactions
- Secondary hit validation